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Journal : Edubiotik : Jurnal Pendidikan, Biologi dan Terapan

PROSES REGENERASI SIRIP EKOR PADA IKAN ZEBRA Sari, Nila Kartika; Listyorini, Dwi; Gofur, Abdul
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol 1 No 01 (2016): Edubiotik
Publisher : Program Studi Pendidikan Biologi, IKIP Budi Utomo Malang

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Abstract

The caudal fin of Zebra fish as a model for studies regeneration both in terms of process and study of molecular analysis. This research is a descriptive exploratory. A sample of zebra fish (Danio rerio) 4-6 months of age as much as 30 individuals. The regeneration caudal fin of zebrafish observed by microscope with cutting vertically. The parameters observed were: 1) the time required for the regeneration process, 2) The regeneration process in details covering and, 3) Type of regeneration. Data in the form of the regeneration process ranging from wound healing to the original length obtained are collected in the form of digital photos. The results showed that the time required to regenerate the caudal fin to recover to normal at a temperature of 22-31° C was 22.25 ± 1.63 days for a model of vertical cuts. The regeneration process begins with the events of wound healing (H1) followed by the formation of blastema and will radius (H2) and continued differentiation characterized by the process of angiogenesis (H2). Regenerative development begins with the formation of the fingers and the growth of the peripheral areas tail fins faster than the medial region (H3-H8). End process regeneration in caudal fin of zebrafish are included in the development is the formation of regenerative segment radius starting from the proximal to the distal portion regenerat regenerat (H9 - ± H21). From the results of this research note that caudal fin of zebrafish to regenerate in epimorphosis.
REGENERASI SIRIP KAUDAL IKAN GATUL (Poecilia sp.) YANG MENGALAMI MALFORMASI Iza, Nikmatul; Listyorini, Dwi; Gofur, Abdul
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol 1 No 01 (2016): Edubiotik
Publisher : Program Studi Pendidikan Biologi, IKIP Budi Utomo Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (747.146 KB)

Abstract

Gatul fish in included in the family Poecilidae which is a small fresh water, male is smaller and more colorfull than female. This fish is easy to be taken care in laboratory and has a short reproduction cycle, thus allowing these fish to be one animal model development in the field of biology. Yet, in Indonesia the biological study upon Gatul fish is very limited. The aim of this study is to understand the process of caudal fin regeneration of Gatul fish are malformation (abnormal growth). Caudal fin of Gatul fish were vertically cut in a half of its length. Observation was done using 400x magnification. Picture with taken by camera with digital zooming. The subject of this study with a body length of 2-3 cm. The result shows that regeneration of Gatul caudal fin is started with wound healing in day-1 (D1= 24 hours after amputation) and blastema formation, then stump regeneration begin to grow in D2. Regenerative outgrowth begin in D3 characterized by the formation Blood island and fin rays. Differentiation begin in D6 and extended up to D18. From this research we can conclude that the process is going step by step through wound healing, stump growth, and tissue differentiation, and respectively